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标题：树突状细胞疫苗对人化荷人膀胱癌SCID鼠循环微转移转归影响的研究 |
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作者：米振国 |
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单位：山西省肿瘤医院 |
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稿件编号：20060623205170 |
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摘要：摘要：目的 以人外周血淋巴细胞(hu-PBL)腹腔转输方法构建有效的具备人免疫环境SCID鼠模型，探讨树突状细胞(DCs)疫苗对荷瘤SCID鼠血循环膀胱癌细胞微转移转归的影响。方法 体外侵袭筛选膀胱癌细胞株T24，完成三次筛选的子代细胞记作T24-3；自人外周血密度梯度离心分离hu-PBL，4×107/鼠，腹腔注射，1d后取鼠右后肢内侧皮下接种3×106个T24-3细胞，2w、4w、5w、6w不同周次检测鼠体内人免疫状态(包括IgG和CD3+、CD4+、CD8+T细胞)及外周血细胞角蛋白20(CK20) mRNA表达；含10%人AB型血清完全RPMI 1640培养基和rhGM-CSF、rhIL-4，37℃、5%CO2培养体系体外诱导培养人外周血单个核细胞(PBMC)来源DCs，冻融法获得的T24-3细胞裂解上清液致敏DCs；并干预人化荷瘤SCID鼠自发血循环肿瘤细胞微转移，观察大体情况、外周血CK20mRNA表达及瘤体MMP-7mRNA表达。结果 于肿瘤细胞接种后5w进行干预。DCs疫苗组0例CK20mRNA(+)，0例发生远处转移，PBS对照组2例CK20mRNA(+)，3例发生远处转移；MMP-7mRNA在DCs疫苗组表达最低，较PBS对照组差异有显著意义，P=0.0073。结论 DCs疫苗可以有效控制人化荷人膀胱癌SCID鼠血循环肿瘤细胞微转移发生，进而延缓或阻止肿瘤转移。
关键词：树突状细胞，膀胱肿瘤，SCID鼠，免疫重建，微转移
中图分类号：R737.14 文献标识码：A 文章编号：
Effects of Dendritic Cell Vaccines on Hematogenous Micrometastasis of Bladder Cancer Model via Inoculating Human Bladder Transitional Cell Carcinoma Cells into Hu-PBL-SCID Mice
MI Zhen-guo, WANG Bin, LI Zhi-bin, et al. Department of Urology, Shanxi Tumor Hospital, Taiyuan 030013, China
Abstract:Objective To study the effect of dendritic cells loaded with whole tumor antigen on hematogenous micrometastasis of bladder cancer model in hu-PBL-SCID mice. Methods T24-3 cell line isolated from human bladder transitional cell carcinoma T24 cell line by Boyden chamber system. The hu-PBL-T24-3 -SCID model was established via intraperitoneally injected with 4×107 hu-PBL and subcutaneously injected with 3×106 T24-3. The detection of human IgG in blood plasma by EILSA、the detection of human CD3+、CD4+、CD8+T cells in blood and spleen cells by FCM were adopted to study human immune reconstruction; human CK20mRNA(+) in mice peripheral blood by RT-PCR were adopted to investigate metastasis of tumor cells. The PBMCs were isolated from human peripheral blood, and then were induced and cultured in vitro by rhGM-CSF、rhIL-4 and then developed into DCs .The DC vaccines were produced by co-culturing with whole tumor antigen which was purified through freezing and melting T24-3 cell line. In the end of treated ,RT-PCR and fluorescence quantitative RT-PCR were adopted to investigate the effect. Results: All mice were totally treated from 5th week on. The expression of CK20mRNA in DC vaccines group(none) was the lowest, compared with DCs group and PBS group, and there was 2 mice CK20mRNA(+) and 3 mice with metastasis tumor in PBS group. MMP-7 level of DC vaccines group was statistically lower than PBS group, P=0.0073. Conclusion: DC vaccines showed the good effects by reducing the appearance of hematogenous micrometastasis in hu-PBL-SCID mice bladder cancer model.
Key word：dendritic cells, bladder tumor, SCID mouse, immune reconstruction, micrometastasis
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标题：TURP和TUVP治疗前列腺增生症的临床选择(附102例报告) |
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作者：徐勇杰 |
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单位：武警山西总队医院泌尿外科 |
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稿件编号：20060710124270 |
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摘要： [关键词] 前列腺增生症;经尿道前列腺气化术;经尿道前列腺电切术
临床治疗前列腺增生（BPH）的腔镜治疗方法日新月异，由经尿道前列腺电切术（TURP）到第二代经尿道前列腺增生气化术(TUVP)，近年来出现的等离子电切（PK）和经尿道选择性绿激光前列腺汽化术(PVP)，都显示了微创治疗独特的优点，然而就基层医院TURP和TUVP仍然是最为普及和成熟的治疗方法，两种方法的临床的选择应用充分发挥各自特点对缩短手术时间减少手术并发症有较大裨益，现将我院近期临床102例BPH患者微创治疗临床经验总结如下：
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标题：间质性膀胱炎膀胱水囊扩张加西施泰灌注5例报告 |
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作者：王旭 |
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单位：北京航天总医院泌尿外科（王旭）；天津医科 |
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稿件编号：20060711164670 |
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摘要： 间质性膀胱炎是一种原因未明的以尿急、尿频、膀胱充盈后耻骨上区及盆腔疼痛，排尿后减轻为表现的临床综合症。其病因复杂、机制不清、疗效不佳，由于诊断标准的改变，其发病率有明显逐年上升的趋势。间质性膀胱炎的治疗效果历来不佳，本报告对5例病人进行膀胱水囊扩张加西施泰(透明质酸钠)膀胱灌注治疗，半年后症状明显减轻，钾敏感试验阴性、盆腔症状评分处于正常范围, 膀胱疼痛时最大膀胱容量明显增加，疗效满意。
[关键词] 间质性膀胱炎；膀胱水囊扩张；西施泰膀胱灌注 |
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标题：硫酸镁静滴治疗肾绞痛疗效分析 |
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作者：谷建明 |
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单位：甘肃省张掖市甘州区医院泌尿外科 |
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稿件编号：20060712110470 |
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摘要：摘要 目的：探讨肾绞痛治疗方法。方法：2000年8 月至2005年8月收治肾绞痛患者220例随机分为常规组：108例，应用杜冷丁50mg和阿托品0.5mg肌注治疗；硫酸镁组：112例，用25%硫酸镁10ml+10%葡萄糖100ml缓慢静推后再用25%硫酸镁20ml+10%葡萄糖500ml持续静点。单盲对照观察疗效。结果：硫酸镁组治疗肾绞痛缓解时间为17±7分钟，治愈时间为25±9分钟，无效3例，再发作2例；常规组分别为28±10分钟、42±12分钟、18例、28例。结论：硫酸镁治疗肾绞痛临床效果满意，值得推广。
关键词：硫酸镁；肾绞痛 |
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标题：单纯性阴茎下弯的手术治疗 |
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作者：张星 |
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单位：江苏省扬州市中医院 |
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稿件编号：20060714195370 |
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摘要：[摘要]目的：探讨手术治疗单纯性阴茎下弯的方法。方法：在阴茎阴囊交界处采用自创切口，斜形切断尿道，人工勃起后，松解切除阴茎海绵体腹侧表面纤维索带，至阴茎完全伸直，取膀胱粘膜制成粘膜管与尿道近远端斜形吻合。结果：无阴茎短缩、尿瘘、尿道狭窄等并发症，无阴茎下弯复发，外形美观。结论：采用膀胱粘膜管治疗单纯性阴茎下弯是安全有效的方法 |
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